Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
RAD21mAC_OsTIR
cell line
HCT116 cells
treatment
auxin (IAA) treated_depletion

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted from cells, sonicated, pre-cleared and this lysate was used for ChIP libarry prep. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit with 6-8 cycles of PCR.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
37811051
Reads aligned (%)
7.7
Duplicates removed (%)
13.5
Number of peaks
75 (qval < 1E-05)

hg19

Number of total reads
37811051
Reads aligned (%)
7.7
Duplicates removed (%)
13.7
Number of peaks
99 (qval < 1E-05)

Base call quality data from DBCLS SRA