Antigen

Antigen Class

TFs and others

Antigen

RAD21

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

RAD21mAC_OsTIR

cell line

HCT116 cells

treatment

auxin (IAA) treated_depletion

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was extracted from cells, sonicated, pre-cleared and this lysate was used for ChIP libarry prep. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit with 6-8 cycles of PCR.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

37811051

Reads aligned (%)

7.7

Duplicates removed (%)

13.5

Number of peaks

75 (qval < 1E-05)

hg19

Number of total reads

37811051

Reads aligned (%)

7.7

Duplicates removed (%)

13.7

Number of peaks

99 (qval < 1E-05)